Utilization of enzyme extract self-encapsulated within polypyrrole in sensitive detection of catechol.
Identifieur interne : 000192 ( Main/Exploration ); précédent : 000191; suivant : 000193Utilization of enzyme extract self-encapsulated within polypyrrole in sensitive detection of catechol.
Auteurs : Roxana-Mihaela Apetrei [Roumanie] ; Geta Cârâc [Roumanie] ; Gabriela Bahrim [Roumanie] ; Pinar Camurlu [Turquie]Source :
- Enzyme and microbial technology [ 1879-0909 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Catéchols.
- enzymologie : Trametes.
- isolement et purification : Mélanges complexes.
- métabolisme : Catechol oxidase, Enzymes immobilisées, Mélanges complexes.
- méthodes : Techniques de biocapteur.
- Polymères, Pyrroles.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Catechols.
- chemical , isolation & purification : Complex Mixtures.
- chemical , metabolism : Catechol Oxidase, Complex Mixtures, Enzymes, Immobilized.
- enzymology : Trametes.
- methods : Biosensing Techniques.
- chemical : Polymers, Pyrroles.
Abstract
Utilization of polyphenol oxidases (laccase, tyrosinase) in biosensor technology is an efficient approach towards phenol detection, which is significant in numerous fields such as environmental monitoring, food industry etc. The use of crude extract instead of pure enzyme eliminates the need for costly and laborious processes of enzyme separation and purification. This study employs polyphenol oxidase extract, biosynthesized by white-rot fungi Trametes pubescens (TP) for the development of amperometric biosensors for catechol detection. The catalytic activity of the crude extract was firstly used to induce the bio-synthesis of conducting polymer - polypyrrole (Ppy), resulting in the self-encapsulation of the enzyme extract within the conducting material. The viability and biological integrity of the enzyme extract was preserved after the synthesis and was able to efficiently detect phenolic compounds such as catechol. Comparative evaluations between the biosynthesized Ppy based biosensor (bio-Ppy) and the biosensor based on bio-PPy with additional enzyme extract (bio-Ppy-TP) were performed. Lastly, the performance of these two biosensors was compared with that of a third one, based on chemically synthesized Ppy with enzyme extract (chem-Ppy-TP). All three types of biosensors proved high efficiency for catechol detection at low concentration (1-60 μM) and were employed for real sample detection in fruit wines showing linear correlation with the spectrophotometric results obtained with the Folin-Ciocalteau standard test.
DOI: 10.1016/j.enzmictec.2019.04.015
PubMed: 31186108
Affiliations:
Links toward previous steps (curation, corpus...)
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Electronic address: pcamurlu@akdeniz.edu.tr.</nlm:affiliation><country xml:lang="fr">Turquie</country><wicri:regionArea>Akdeniz University, Department of Chemistry, 07058, Antalya</wicri:regionArea><wicri:noRegion>Antalya</wicri:noRegion></affiliation></author></analytic><series><title level="j">Enzyme and microbial technology</title><idno type="eISSN">1879-0909</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques (methods)</term><term>Catechol Oxidase (metabolism)</term><term>Catechols (analysis)</term><term>Complex Mixtures (isolation & purification)</term><term>Complex Mixtures (metabolism)</term><term>Enzymes, Immobilized (metabolism)</term><term>Polymers (MeSH)</term><term>Pyrroles (MeSH)</term><term>Trametes (enzymology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Catechol oxidase (métabolisme)</term><term>Catéchols (analyse)</term><term>Enzymes immobilisées (métabolisme)</term><term>Mélanges complexes (isolement et purification)</term><term>Mélanges complexes (métabolisme)</term><term>Polymères (MeSH)</term><term>Pyrroles (MeSH)</term><term>Techniques de biocapteur (méthodes)</term><term>Trametes (enzymologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Catechols</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Complex Mixtures</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Catechol Oxidase</term><term>Complex Mixtures</term><term>Enzymes, Immobilized</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Catéchols</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Trametes</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Trametes</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Mélanges complexes</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Catechol oxidase</term><term>Enzymes immobilisées</term><term>Mélanges complexes</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Techniques de biocapteur</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Polymers</term><term>Pyrroles</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Polymères</term><term>Pyrroles</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Utilization of polyphenol oxidases (laccase, tyrosinase) in biosensor technology is an efficient approach towards phenol detection, which is significant in numerous fields such as environmental monitoring, food industry etc. The use of crude extract instead of pure enzyme eliminates the need for costly and laborious processes of enzyme separation and purification. This study employs polyphenol oxidase extract, biosynthesized by white-rot fungi Trametes pubescens (TP) for the development of amperometric biosensors for catechol detection. The catalytic activity of the crude extract was firstly used to induce the bio-synthesis of conducting polymer - polypyrrole (Ppy), resulting in the self-encapsulation of the enzyme extract within the conducting material. The viability and biological integrity of the enzyme extract was preserved after the synthesis and was able to efficiently detect phenolic compounds such as catechol. Comparative evaluations between the biosynthesized Ppy based biosensor (bio-Ppy) and the biosensor based on bio-PPy with additional enzyme extract (bio-Ppy-TP) were performed. Lastly, the performance of these two biosensors was compared with that of a third one, based on chemically synthesized Ppy with enzyme extract (chem-Ppy-TP). All three types of biosensors proved high efficiency for catechol detection at low concentration (1-60 μM) and were employed for real sample detection in fruit wines showing linear correlation with the spectrophotometric results obtained with the Folin-Ciocalteau standard test.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31186108</PMID><DateCompleted><Year>2019</Year><Month>11</Month><Day>08</Day></DateCompleted><DateRevised><Year>2019</Year><Month>11</Month><Day>08</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0909</ISSN><JournalIssue CitedMedium="Internet"><Volume>128</Volume><PubDate><Year>2019</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Enzyme and microbial technology</Title><ISOAbbreviation>Enzyme Microb Technol</ISOAbbreviation></Journal><ArticleTitle>Utilization of enzyme extract self-encapsulated within polypyrrole in sensitive detection of catechol.</ArticleTitle><Pagination><MedlinePgn>34-39</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0141-0229(19)30065-1</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.enzmictec.2019.04.015</ELocationID><Abstract><AbstractText>Utilization of polyphenol oxidases (laccase, tyrosinase) in biosensor technology is an efficient approach towards phenol detection, which is significant in numerous fields such as environmental monitoring, food industry etc. The use of crude extract instead of pure enzyme eliminates the need for costly and laborious processes of enzyme separation and purification. This study employs polyphenol oxidase extract, biosynthesized by white-rot fungi Trametes pubescens (TP) for the development of amperometric biosensors for catechol detection. The catalytic activity of the crude extract was firstly used to induce the bio-synthesis of conducting polymer - polypyrrole (Ppy), resulting in the self-encapsulation of the enzyme extract within the conducting material. The viability and biological integrity of the enzyme extract was preserved after the synthesis and was able to efficiently detect phenolic compounds such as catechol. Comparative evaluations between the biosynthesized Ppy based biosensor (bio-Ppy) and the biosensor based on bio-PPy with additional enzyme extract (bio-Ppy-TP) were performed. Lastly, the performance of these two biosensors was compared with that of a third one, based on chemically synthesized Ppy with enzyme extract (chem-Ppy-TP). All three types of biosensors proved high efficiency for catechol detection at low concentration (1-60 μM) and were employed for real sample detection in fruit wines showing linear correlation with the spectrophotometric results obtained with the Folin-Ciocalteau standard test.</AbstractText><CopyrightInformation>Copyright © 2019 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Apetrei</LastName><ForeName>Roxana-Mihaela</ForeName><Initials>RM</Initials><AffiliationInfo><Affiliation>Dunărea de Jos University of Galati, Faculty of Food Science and Engineering, Domnească Street, 47, RO-800008, Galati, Romania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cârâc</LastName><ForeName>Geta</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Dunărea de Jos University of Galati, Faculty of Science and Environment, Domnească Street, 47, RO-800008, Galati, Romania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bahrim</LastName><ForeName>Gabriela</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Dunărea de Jos University of Galati, Faculty of Food Science and Engineering, Domnească Street, 47, RO-800008, Galati, Romania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Camurlu</LastName><ForeName>Pinar</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Akdeniz University, Department of Chemistry, 07058, Antalya, Turkey. Electronic address: pcamurlu@akdeniz.edu.tr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>05</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Enzyme Microb Technol</MedlineTA><NlmUniqueID>8003761</NlmUniqueID><ISSNLinking>0141-0229</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002396">Catechols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D045424">Complex Mixtures</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004800">Enzymes, Immobilized</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011108">Polymers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011758">Pyrroles</NameOfSubstance></Chemical><Chemical><RegistryNumber>30604-81-0</RegistryNumber><NameOfSubstance UI="C067635">polypyrrole</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.10.3.1</RegistryNumber><NameOfSubstance UI="D004156">Catechol Oxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>LF3AJ089DQ</RegistryNumber><NameOfSubstance UI="C034221">catechol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015374" MajorTopicYN="N">Biosensing Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004156" MajorTopicYN="N">Catechol Oxidase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002396" MajorTopicYN="N">Catechols</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045424" MajorTopicYN="N">Complex Mixtures</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004800" MajorTopicYN="N">Enzymes, Immobilized</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011108" MajorTopicYN="N">Polymers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011758" MajorTopicYN="N">Pyrroles</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055454" MajorTopicYN="N">Trametes</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Amperometric biosensors</Keyword><Keyword MajorTopicYN="N">Crude enzyme extract</Keyword><Keyword MajorTopicYN="N">Ppy biosynthesis</Keyword><Keyword MajorTopicYN="N">Sensitive catechol detection</Keyword><Keyword MajorTopicYN="N">Trametes pubescens</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>02</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>04</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>04</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>6</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>6</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>11</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31186108</ArticleId><ArticleId IdType="pii">S0141-0229(19)30065-1</ArticleId><ArticleId IdType="doi">10.1016/j.enzmictec.2019.04.015</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Roumanie</li><li>Turquie</li></country></list><tree><country name="Roumanie"><noRegion><name sortKey="Apetrei, Roxana Mihaela" sort="Apetrei, Roxana Mihaela" uniqKey="Apetrei R" first="Roxana-Mihaela" last="Apetrei">Roxana-Mihaela Apetrei</name></noRegion><name sortKey="Bahrim, Gabriela" sort="Bahrim, Gabriela" uniqKey="Bahrim G" first="Gabriela" last="Bahrim">Gabriela Bahrim</name><name sortKey="Carac, Geta" sort="Carac, Geta" uniqKey="Carac G" first="Geta" last="Cârâc">Geta Cârâc</name></country><country name="Turquie"><noRegion><name sortKey="Camurlu, Pinar" 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